Pay attention to the suitability of choosing ppe

Identification

Ordinary authentication

(1) The product powder is light green or yellowish green. Crystal fiber, calcium oxalate crystal diameter 12 ~ 15μm.

Non-glandular single cells, 100-350 μm in length, 12-25 μm in diameter, with thick walls and verrucous processes. Calcium oxalate clusters exist in mesophyll parenchyma cells and have a diameter of 9-20 μm. The surface of the upper and lower epidermal cells was polygonal, and the vertical wall was straight; both the upper and lower epidermis had stomata, the main axis was flat, and there were mostly 2 sub-advanced cells and 3 of them. (2) Take the product powder 25mg, add water 50ml and hydrochloric acid 2ml, set in a water bath and heat for 15 minutes, let cool, add ether 40ml, shake to extract, take the ether layer, dehydrate through the anhydrous sodium sulfate layer, filter, take Filtrate 5ml, evaporated to dryness, let cool, add ammonia test solution 5ml, the solution was yellow or orange, set in a water bath and heated for 2 minutes, turned purple.

(3) Take 0.5g of this product powder, add 3ml of equal mixture solution of ethanol and water, sonicate for 30 minutes, centrifuge, and aspirate the supernatant as test solution. In addition, the reference material for the scented cilia was 0. 5g, and the reference drug solution was prepared with the same method. According to the thin layer chromatography (Appendix VI B) test, 10 μl of each of the above two solutions was pipetted and spotted on the same silica gel G thin plate in strips with ethyl acetate-n-propanol-water (4:4) : 3) For developer, unfold, remove, dry, and view under UV light (365nm). In the chromatogram of the test sample, fluorescent spots of the same color appear on the positions corresponding to the chromatograms of the control herbs; sprayed with 20% nitric acid solution and added at 120°C.

Heat about 10 minutes, let cool, then spray with 5% potassium hydroxide in dilute ethanol solution and examine under sunlight. In the chromatogram of the test sample, spots of the same color were observed at the positions corresponding to the chromatogram of the reference drug.

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